Applications of Flow Cytometry in Stem Cell Research and by Awtar Krishan,H. Krishnamurthy,Satish Totey

By Awtar Krishan,H. Krishnamurthy,Satish Totey

A much-needed primer at the use of laser move cytometry for stem mobilephone analysis

Laser circulation cytometry is a strong instrument for swift research of cells for marker expression, cellphone cycle place, proliferation, and apoptosis. even though, no assets particularly tackle using this system for the examine of stem cells; this is often in particular vital as stem telephone research consists of really good tools and marking strategies in keeping with particular features reminiscent of marker expression, phone dimension, drug shipping, and efflux of the stem cells.

Now, this publication reports those techniques, discusses the technological know-how in the back of them, and offers real-world examples to demonstrate the usefulness of the equipment. It brings jointly world-class specialists in pathology, biophysics, immunology, and stem phone examine, who draw upon their broad event with the equipment and convey examples of excellent info to assist advisor researchers within the correct direction.

Chapter assurance includes:

  • Stem cellphone research and sorting utilizing part population
  • Flow cytometry within the research of proliferation and apoptosis
  • Stem mobilephone biology and application
  • Identification and isolation of very small embryonic-like stem cells from murine and human specimens
  • Hematopoietic stem cells—issues in enumeration
  • Human embryonic stem cells: long term tradition and cardiovascular differentiation
  • Limbal stem cells and corneal regeneration
  • Flow cytometric sorting of spermatogonial stem cells
  • Breast melanoma stem cells
  • Stem mobilephone marker expression in cells from physique hollow space fluids

This booklet is a necessary source for all graduate scholars, practitioners in constructing nations, libraries and publication repositories of universities and learn associations, and person researchers. it's also of curiosity to laboratories engaged in stem mobilephone study and use of stem cells for tissue regeneration, and to any association dealing in stem mobilephone and tissue regeneration research.

Show description

Read Online or Download Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration PDF

Best cell biology books

International Review of Cell and Molecular Biology: 267

Overseas evaluation of telephone and Molecular Biology provides present advances and complete stories in cellphone biology—both plant and animal. Articles deal with constitution and keep an eye on of gene expression, nucleocytoplasmic interactions, regulate of phone improvement and differentiation, and cellphone transformation and development.

Deep Metazoan Phylogeny: The Backbone of the Tree of Life: New insights from analyses of molecules, morphology, and theory of data analysis

The starting to be good fortune of molecular tools has challenged conventional perspectives of animal evolution and plenty of substitute hypotheses are hotly debated at the present time. For the deep metazoan phylogeny venture, information units of hitherto unrivaled caliber and volume have been compiled and analysed with leading edge bioinformatics instruments.

Cancer Stem Cells

A brand new healing method may possibly holiday the stalemate within the warfare on melanoma through focusing on now not all cancerous cells however the small fraction that lie on the root of cancers. Lucie Laplane deals a complete research of melanoma stem telephone thought, according to an unique interdisciplinary procedure that mixes biology, biomedical background, and philosophy.

Lysosomes: Biology, Diseases, and Therapeutics

Discussing contemporary findings, updated examine, and novel innovations, the publication integrates views from pharmacology, toxicology, and biochemistry to demonstrate the opportunity of lysosomes in drug discovery and improvement. •    Explores uncomplicated rules and homes of lysosomes that let them to behave as regulators of telephone metabolism, healing goals, and websites for activation of drug conjugates •    Discusses the function of lysosomes in metabolism, drug concentrating on, apoptosis, melanoma, getting older, irritation, autophagy, metabolism, toxicity, and membrane repair•    Introduces new pathways in healing improvement and new mechanisms in drug improvement

Extra info for Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

Example text

Download PDF sample

Rated 4.55 of 5 – based on 43 votes