By Awtar Krishan,H. Krishnamurthy,Satish Totey
Laser circulation cytometry is a strong instrument for swift research of cells for marker expression, cellphone cycle place, proliferation, and apoptosis. even though, no assets particularly tackle using this system for the examine of stem cells; this is often in particular vital as stem telephone research consists of really good tools and marking strategies in keeping with particular features reminiscent of marker expression, phone dimension, drug shipping, and efflux of the stem cells.
Now, this publication reports those techniques, discusses the technological know-how in the back of them, and offers real-world examples to demonstrate the usefulness of the equipment. It brings jointly world-class specialists in pathology, biophysics, immunology, and stem phone examine, who draw upon their broad event with the equipment and convey examples of excellent info to assist advisor researchers within the correct direction.
Chapter assurance includes:
- Stem cellphone research and sorting utilizing part population
- Flow cytometry within the research of proliferation and apoptosis
- Stem mobilephone biology and application
- Identification and isolation of very small embryonic-like stem cells from murine and human specimens
- Hematopoietic stem cells—issues in enumeration
- Human embryonic stem cells: long term tradition and cardiovascular differentiation
- Limbal stem cells and corneal regeneration
- Flow cytometric sorting of spermatogonial stem cells
- Breast melanoma stem cells
- Stem mobilephone marker expression in cells from physique hollow space fluids
This booklet is a necessary source for all graduate scholars, practitioners in constructing nations, libraries and publication repositories of universities and learn associations, and person researchers. it's also of curiosity to laboratories engaged in stem mobilephone study and use of stem cells for tissue regeneration, and to any association dealing in stem mobilephone and tissue regeneration research.
Read Online or Download Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration PDF
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